Genotyping and primer details


Genotyping and primer details for 313A06 - At2g23950
Line ID 313A06
Locus At2g23950 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:10189290 of line 313A06 Chr2:10189290
   
Insertion allele amplicon 8474 - EQ19
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer EQ19: 5'-TCTCTCTCTTCTGAACCCAGAAAC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:10189897
   
Insertion allele amplicon 8474 - KH52
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer KH52: 5'-TAGGAACAGGACCTCTGAGATTGT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:10188960
   
wt amplicon (over InsPos) KH52 - EQ19
wt amplicon start Chr2:10188960
wt amplicon end Chr2:10189897
wt amplicon size 938 bp
Locus specific primer EQ19: 5'-TCTCTCTCTTCTGAACCCAGAAAC-3'
2nd locus specific primer KH52: 5'-TAGGAACAGGACCTCTGAGATTGT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11