Genotyping and primer details


Genotyping and primer details for 320E07 - At1g48500
Line ID 320E07
Locus At1g48500 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:17932373 of line 320E07 Chr1:17932373
   
Insertion allele amplicon 8474 - 15vd
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 15vd: 5'-GAGAATGACCAATCCATCATACCT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:17933013
   
wt amplicon (over InsPos) 70vo - 15vd
wt amplicon start Chr1:17932044
wt amplicon end Chr1:17933013
wt amplicon size 970 bp
Locus specific primer 15vd: 5'-GAGAATGACCAATCCATCATACCT-3'
2nd locus specific primer 70vo: 5'-ACGTTTTGGGACTCGATAGTTAGA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11