Genotyping and primer details


Genotyping and primer details for 323A02 - At2g39430
Line ID 323A02
Locus At2g39430 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:16464182 of line 323A02 Chr2:16464182
   
Insertion allele amplicon 8474 - KS80
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer KS80: 5'-ACATCAACAGTGTCATTAACCCAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:16463840
   
wt amplicon (over InsPos) 23si - KS80
wt amplicon start Chr2:16463840
wt amplicon end Chr2:16464583
wt amplicon size 744 bp
Locus specific primer KS80: 5'-ACATCAACAGTGTCATTAACCCAA-3'
2nd locus specific primer 23si: 5'-TACCCATTTGTCAAAGTGTTTCAA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11