Genotyping and primer details


Genotyping and primer details for 323F09 - At3g47720
Line ID 323F09
Locus At3g47720 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:17593321 of line 323F09 Chr3:17593321
   
Insertion allele amplicon 8474 - 35sm
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 35sm: 5'-TCTCCAGTTATACTTCTCAGGCGT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:17593908
   
wt amplicon (over InsPos) 15ti - 35sm
wt amplicon start Chr3:17592866
wt amplicon end Chr3:17593908
wt amplicon size 1043 bp
Locus specific primer 35sm: 5'-TCTCCAGTTATACTTCTCAGGCGT-3'
2nd locus specific primer 15ti: 5'-TTGTGATCAACAACATTCTTCCAT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11