Genotyping and primer details


Genotyping and primer details for 324H01 - At1g71770
Line ID 324H01
Locus At1g71770 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:26992275 of line 324H01 Chr1:26992275
   
Insertion allele amplicon 8474 - 24bk
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 24bk: 5'-AAACACGTGAAAACATCAAGACAG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:26991751
   
wt amplicon (over InsPos) 04cm - 24bk
wt amplicon start Chr1:26991751
wt amplicon end Chr1:26992360
wt amplicon size 610 bp
Locus specific primer 24bk: 5'-AAACACGTGAAAACATCAAGACAG-3'
2nd locus specific primer 04cm: 5'-GAAGAGGAATTGAGGAGGAAGTTT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11