Genotyping and primer details


Genotyping and primer details for 325C01 - At3g45590
Line ID 325C01
Locus At3g45590 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:16733533 of line 325C01 Chr3:16733533
   
Insertion allele amplicon 8474 - BV31
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer BV31: 5'-GCAGCTTATAGGAATGTCAAGAGG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:16734065
   
wt amplicon (over InsPos) 07nv - BV31
wt amplicon start Chr3:16733161
wt amplicon end Chr3:16734065
wt amplicon size 905 bp
Locus specific primer BV31: 5'-GCAGCTTATAGGAATGTCAAGAGG-3'
2nd locus specific primer 07nv: 5'-GAGGCTTTAGGTTTCCTCTCAAGT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11