Genotyping and primer details


Genotyping and primer details for 325F01 - At1g63110
Line ID 325F01
Locus At1g63110 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:23404850 of line 325F01 Chr1:23404850
   
Insertion allele amplicon 8474 - 01tj
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 01tj: 5'-CTACCTTTGAACTGTGAGAGGTCC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:23405302
   
wt amplicon (over InsPos) 08tx - 01tj
wt amplicon start Chr1:23404337
wt amplicon end Chr1:23405302
wt amplicon size 966 bp
Locus specific primer 01tj: 5'-CTACCTTTGAACTGTGAGAGGTCC-3'
2nd locus specific primer 08tx: 5'-AGAAATCAAAAACTGCCCTAGACA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11