Genotyping and primer details


Genotyping and primer details for 329B06 - At5g35730
Line ID 329B06
Locus At5g35730 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:13894973 of line 329B06 Chr5:13894973
   
Insertion allele amplicon 8474 - PF26
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer PF26: 5'-AAGAGCAGTAGTTGGCCAATAAAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:13895493
   
wt amplicon (over InsPos) 22sq - PF26
wt amplicon start Chr5:13894564
wt amplicon end Chr5:13895493
wt amplicon size 930 bp
Locus specific primer PF26: 5'-AAGAGCAGTAGTTGGCCAATAAAA-3'
2nd locus specific primer 22sq: 5'-CTTATGGGTGTTCTCTCAAGGAAG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11