Genotyping and primer details


Genotyping and primer details for 330C06 - At4g35670
Line ID 330C06
Locus At4g35670 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:16916775 of line 330C06 Chr4:16916775
   
Insertion allele amplicon 8474 - SD24
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer SD24: 5'-ATTGAAAACGTGGGTAAACTTTTG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:16917246
   
wt amplicon (over InsPos) 11dw - SD24
wt amplicon start Chr4:16916093
wt amplicon end Chr4:16917246
wt amplicon size 1154 bp
Locus specific primer SD24: 5'-ATTGAAAACGTGGGTAAACTTTTG-3'
2nd locus specific primer 11dw: 5'-AATCTTTTTGCACCTGAGACAAGT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11