Genotyping and primer details


Genotyping and primer details for 333B07 - At3g05170
Line ID 333B07
Locus At3g05170 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:1466760 of line 333B07 Chr3:1466760
   
Insertion allele amplicon 8474 - 16us
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 16us: 5'-AAACAAAAGAGAGATGGTGGTTTG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:1466202
   
wt amplicon (over InsPos) 45vc - 16us
wt amplicon start Chr3:1466202
wt amplicon end Chr3:1467122
wt amplicon size 921 bp
Locus specific primer 16us: 5'-AAACAAAAGAGAGATGGTGGTTTG-3'
2nd locus specific primer 45vc: 5'-CAAATCTCTCTCTGACCTTTTTCG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11