Genotyping and primer details


Genotyping and primer details for 336E01 - At4g29800
Line ID 336E01
Locus At4g29800 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:14591214 of line 336E01 Chr4:14591214
   
Insertion allele amplicon 8474 - 25ay
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 25ay: 5'-TGCAAGAAACAGGTCTCACTCTAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:14590600
   
wt amplicon (over InsPos) 10cm - 25ay
wt amplicon start Chr4:14590600
wt amplicon end Chr4:14591811
wt amplicon size 1212 bp
Locus specific primer 25ay: 5'-TGCAAGAAACAGGTCTCACTCTAA-3'
2nd locus specific primer 10cm: 5'-GCTAGAAAAAGCAATGAAAGCATC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11