Genotyping and primer details


Genotyping and primer details for 337E11 - At4g30980
Line ID 337E11
Locus At4g30980 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:15079528 of line 337E11 Chr4:15079528
   
Insertion allele amplicon 8474 - 18uv
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 18uv: 5'-CGAGTTTTAAACAAACTCTTTGGC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:15079162
   
wt amplicon (over InsPos) 58vd - 18uv
wt amplicon start Chr4:15079162
wt amplicon end Chr4:15079856
wt amplicon size 695 bp
Locus specific primer 18uv: 5'-CGAGTTTTAAACAAACTCTTTGGC-3'
2nd locus specific primer 58vd: 5'-TCATCATAGCTTTAGGGTTTTGCT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11