Genotyping and primer details


Genotyping and primer details for 340F02 - At5g56060
Line ID 340F02
Locus At5g56060 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:22704643 of line 340F02 Chr5:22704643
   
Insertion allele amplicon 8474 - CL87
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer CL87: 5'-GCAACAATATAGCAACAAGCAAAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:22704034
   
Insertion allele amplicon 8474 - CL88
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer CL88: 5'-TGTCTCTTTACTGGTCTCGTGCTA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:22705117
   
wt amplicon (over InsPos) CL88 - CL87
wt amplicon start Chr5:22704034
wt amplicon end Chr5:22705117
wt amplicon size 1083 bp
Locus specific primer CL87: 5'-GCAACAATATAGCAACAAGCAAAA-3'
2nd locus specific primer CL88: 5'-TGTCTCTTTACTGGTCTCGTGCTA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11