Genotyping and primer details


Genotyping and primer details for 343H07 - At1g55750
Line ID 343H07
Locus At1g55750 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:20841907 of line 343H07 Chr1:20841907
   
Insertion allele amplicon 8474 - 17qk
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 17qk: 5'-ATTGACATTGTGCTCTTTGTATGC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:20842467
   
wt amplicon (over InsPos) 26rd - 17qk
wt amplicon start Chr1:20841342
wt amplicon end Chr1:20842467
wt amplicon size 1126 bp
Locus specific primer 17qk: 5'-ATTGACATTGTGCTCTTTGTATGC-3'
2nd locus specific primer 26rd: 5'-TGTCACTTAGGACACTGGAAAATG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11