Genotyping and primer details


Genotyping and primer details for 344E11 - At2g22460
Line ID 344E11
Locus At2g22460 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:9533367 of line 344E11 Chr2:9533367
   
Insertion allele amplicon 8474 - 48ra
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 48ra: 5'-GCCGAATTATTCTCATTTTGTCTC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:9533075
   
wt amplicon (over InsPos) 06rt - 48ra
wt amplicon start Chr2:9533075
wt amplicon end Chr2:9533729
wt amplicon size 655 bp
Locus specific primer 48ra: 5'-GCCGAATTATTCTCATTTTGTCTC-3'
2nd locus specific primer 06rt: 5'-GGTTTTAGCTCGAGAAATGAGAGA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11