Genotyping and primer details


Genotyping and primer details for 345B11 - At5g46240
Line ID 345B11
Locus At5g46240 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:18745362 of line 345B11 Chr5:18745362
   
Insertion allele amplicon 8474 - 22kh
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 22kh: 5'-AGCCTCTTGTTTAACTACAACGGA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:18745869
   
wt amplicon (over InsPos) 08ky - 22kh
wt amplicon start Chr5:18744929
wt amplicon end Chr5:18745869
wt amplicon size 941 bp
Locus specific primer 22kh: 5'-AGCCTCTTGTTTAACTACAACGGA-3'
2nd locus specific primer 08ky: 5'-TGTATGTCATGTGGGAGTTGATTT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11