Genotyping and primer details


Genotyping and primer details for 346A05 - At1g01470
Line ID 346A05
Locus At1g01470 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:172373 of line 346A05 Chr1:172373
   
Insertion allele amplicon 8474 - 19qz
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 19qz: 5'-GCAGAATTAAAATGGAAACCGATA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:172033
   
wt amplicon (over InsPos) 03rp - 19qz
wt amplicon start Chr1:172033
wt amplicon end Chr1:172655
wt amplicon size 623 bp
Locus specific primer 19qz: 5'-GCAGAATTAAAATGGAAACCGATA-3'
2nd locus specific primer 03rp: 5'-TGTGAGATCAGTTTCACTTTCCAC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11