Genotyping and primer details


Genotyping and primer details for 346H07 - At5g59240
Line ID 346H07
Locus At5g59240 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:23903490 of line 346H07 Chr5:23903490
   
Insertion allele amplicon 8474 - 10kn
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 10kn: 5'-GAGCAGCATCAACTTGAACAATAG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:23903035
   
wt amplicon (over InsPos) 13le - 10kn
wt amplicon start Chr5:23903035
wt amplicon end Chr5:23904058
wt amplicon size 1024 bp
Locus specific primer 10kn: 5'-GAGCAGCATCAACTTGAACAATAG-3'
2nd locus specific primer 13le: 5'-TTAAAACACGAGCAGAAAGTACGA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11