Genotyping and primer details


Genotyping and primer details for 347C07 - At3g50840
Line ID 347C07
Locus At3g50840 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:18897185 of line 347C07 Chr3:18897185
   
Insertion allele amplicon 8474 - 17vc
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 17vc: 5'-ACAAGCATTCTTTCTCTAGCTGGT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:18896523
   
Insertion allele amplicon 8474 - 40vk
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 40vk: 5'-CCTCCAATATCCATGAATGAGTCT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:18896817
   
Insertion allele amplicon 3144 - 91wr
T-DNA primer 3144: 5'-GTGGATTGATGTGATATCTCC-3'
Locus specific primer 91wr: 5'-CCGAGTATCTAGAAATGACGGAAG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:18897916
   
Insertion allele amplicon 3144 - CT18
T-DNA primer 3144: 5'-GTGGATTGATGTGATATCTCC-3'
Locus specific primer CT18: 5'-TGTGATCTTATCCATGAAGTCGAG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:18897576
   
wt amplicon (over InsPos) CT18 - 17vc
wt amplicon start Chr3:18896523
wt amplicon end Chr3:18897576
wt amplicon size 1053 bp
Locus specific primer 17vc: 5'-ACAAGCATTCTTTCTCTAGCTGGT-3'
2nd locus specific primer CT18: 5'-TGTGATCTTATCCATGAAGTCGAG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11