Genotyping and primer details


Genotyping and primer details for 350C02 - At1g59650
Line ID 350C02
Locus At1g59650 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:21920467 of line 350C02 Chr1:21920467
   
Insertion allele amplicon 8474 - 03au
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 03au: 5'-TACTGGATTTGAAGATCGTGTCTG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:21919877
   
Insertion allele amplicon 8474 - AB58
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer AB58: 5'-GACGAAGCAAAAGAATGTTAAAGC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:21920852
   
wt amplicon (over InsPos) 03au - AB58
wt amplicon start Chr1:21919877
wt amplicon end Chr1:21920852
wt amplicon size 976 bp
Locus specific primer AB58: 5'-GACGAAGCAAAAGAATGTTAAAGC-3'
2nd locus specific primer 03au: 5'-TACTGGATTTGAAGATCGTGTCTG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11