Genotyping and primer details


Genotyping and primer details for 350G06 - At4g26910
Line ID 350G06
Locus At4g26910 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:13521296 of line 350G06 Chr4:13521296
   
Insertion allele amplicon 8474 - 25nt
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 25nt: 5'-TTGCACAAATTGAAACTGATAAGG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:13521893
   
wt amplicon (over InsPos) 05oe - 25nt
wt amplicon start Chr4:13520968
wt amplicon end Chr4:13521893
wt amplicon size 926 bp
Locus specific primer 25nt: 5'-TTGCACAAATTGAAACTGATAAGG-3'
2nd locus specific primer 05oe: 5'-GGAGCTTCATCAAATTTGTCCTAC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11