Genotyping and primer details


Genotyping and primer details for 351D08 - At1g04880
Line ID 351D08
Locus At1g04880 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:1378261 of line 351D08 Chr1:1378261
   
Insertion allele amplicon 8474 - 27bk
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 27bk: 5'-TACAATACATTACCCCCAAGAAGC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:1377824
   
wt amplicon (over InsPos) 02cg - 27bk
wt amplicon start Chr1:1377824
wt amplicon end Chr1:1378579
wt amplicon size 756 bp
Locus specific primer 27bk: 5'-TACAATACATTACCCCCAAGAAGC-3'
2nd locus specific primer 02cg: 5'-ATAGCTTTTTCCTTGAAGCCTTTC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11