Genotyping and primer details


Genotyping and primer details for 355B11 - At5g54400
Line ID 355B11
Locus At5g54400 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:22090650 of line 355B11 Chr5:22090650
   
Insertion allele amplicon 8474 - JZ35
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer JZ35: 5'-ACCCGTTAATCACGCAATATAGTC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:22091287
   
wt amplicon (over InsPos) 17gl - JZ35
wt amplicon start Chr5:22090332
wt amplicon end Chr5:22091287
wt amplicon size 956 bp
Locus specific primer JZ35: 5'-ACCCGTTAATCACGCAATATAGTC-3'
2nd locus specific primer 17gl: 5'-GTCACTCACCAAACAAACAACCTA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11