Genotyping and primer details


Genotyping and primer details for 356B08 - At5g63490
Line ID 356B08
Locus At5g63490 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:25419069 of line 356B08 Chr5:25419069
   
Insertion allele amplicon 8474 - CF86
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer CF86: 5'-AGCCCTTGTTCTGTTTGAGTTTTA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:25418456
   
wt amplicon (over InsPos) CF87 - CF86
wt amplicon start Chr5:25418456
wt amplicon end Chr5:25419634
wt amplicon size 1179 bp
Locus specific primer CF86: 5'-AGCCCTTGTTCTGTTTGAGTTTTA-3'
2nd locus specific primer CF87: 5'-TGAGGACTCGAGAAGGTTATTAGC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11