Genotyping and primer details


Genotyping and primer details for 357C12 - At5g14650
Line ID 357C12
Locus At5g14650 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:4725526 of line 357C12 Chr5:4725526
   
Insertion allele amplicon 8474 - CW45
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer CW45: 5'-GTGTATACTGGAAAGGAAACCGAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:4724603
   
wt amplicon (over InsPos) 16vb - CW45
wt amplicon start Chr5:4724603
wt amplicon end Chr5:4725920
wt amplicon size 1318 bp
Locus specific primer CW45: 5'-GTGTATACTGGAAAGGAAACCGAA-3'
2nd locus specific primer 16vb: 5'-ACAGAAAGTTGTCAGCATCTTTGA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11