Genotyping and primer details


Genotyping and primer details for 357D09 - At2g24150
Line ID 357D09
Locus At2g24150 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:10266132 of line 357D09 Chr2:10266132
   
Insertion allele amplicon 8474 - 23sa
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 23sa: 5'-TTCTTCTCTCTTGAGCTTTGAACC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:10266419
   
wt amplicon (over InsPos) 38sq - 23sa
wt amplicon start Chr2:10265657
wt amplicon end Chr2:10266419
wt amplicon size 763 bp
Locus specific primer 23sa: 5'-TTCTTCTCTCTTGAGCTTTGAACC-3'
2nd locus specific primer 38sq: 5'-CGTGAAAAATCAATGATGAGAAGA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11