Genotyping and primer details


Genotyping and primer details for 357G03 - At3g56680
Line ID 357G03
Locus At3g56680 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:20991326 of line 357G03 Chr3:20991326
   
Insertion allele amplicon 8474 - EP35
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer EP35: 5'-AGACTGTATATTTGACGGTTTGGG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:18380597
   
wt amplicon (over InsPos) 25si - EP35
wt amplicon start Chr1:18380597
wt amplicon end Chr3:20991748
wt amplicon size 1135 bp
Locus specific primer EP35: 5'-AGACTGTATATTTGACGGTTTGGG-3'
2nd locus specific primer 25si: 5'-ATCCAGATGACAAAAATCAGCTCT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11