Genotyping and primer details


Genotyping and primer details for 359F04 - At1g29240
Line ID 359F04
Locus At1g29240 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:10218406 of line 359F04 Chr1:10218406
   
Insertion allele amplicon 8474 - 16ve
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 16ve: 5'-TTCAGAGTTGAACTTCTTCCCATT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:10217798
   
wt amplicon (over InsPos) 46vx - 16ve
wt amplicon start Chr1:10217798
wt amplicon end Chr1:10218802
wt amplicon size 1005 bp
Locus specific primer 16ve: 5'-TTCAGAGTTGAACTTCTTCCCATT-3'
2nd locus specific primer 46vx: 5'-AAGTGAATGTCCTTCTGTTCCAGT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11