Genotyping and primer details


Genotyping and primer details for 363G01 - At1g15040
Line ID 363G01
Locus At1g15040 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:5180889 of line 363G01 Chr1:5180889
   
Insertion allele amplicon 8474 - 06bn
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 06bn: 5'-AAGTACGTGGACTTCGTAGGAGAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:5181237
   
wt amplicon (over InsPos) 20cu - 06bn
wt amplicon start Chr1:5180396
wt amplicon end Chr1:5181237
wt amplicon size 842 bp
Locus specific primer 06bn: 5'-AAGTACGTGGACTTCGTAGGAGAA-3'
2nd locus specific primer 20cu: 5'-TCTAGAAACTCAGCTTGGGAGAAG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11