Genotyping and primer details


Genotyping and primer details for 364A11 - At2g45950
Line ID 364A11
Locus At2g45950 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:18904743 of line 364A11 Chr2:18904743
   
Insertion allele amplicon 8474 - BW51
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer BW51: 5'-AACGTAGAACGTGGGTTTACAAAG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:18904428
   
wt amplicon (over InsPos) 29sq - BW51
wt amplicon start Chr2:18904428
wt amplicon end Chr2:18905070
wt amplicon size 643 bp
Locus specific primer BW51: 5'-AACGTAGAACGTGGGTTTACAAAG-3'
2nd locus specific primer 29sq: 5'-GAAGATGACATTTCTACGCCAAAT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11