Genotyping and primer details


Genotyping and primer details for 368G06 - At5g39430
Line ID 368G06
Locus At5g39430 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:15776391 of line 368G06 Chr5:15776391
   
Insertion allele amplicon 3144 - 01ax
T-DNA primer 3144: 5'-GTGGATTGATGTGATATCTCC-3'
Locus specific primer 01ax: 5'-GAGAAGATCATGTGATGGTGAAGA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:15776775
   
Insertion allele amplicon 8474 - 23aq
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 23aq: 5'-GATGCTGTCTTGGTATTGTTGAGA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:15776025
   
wt amplicon (over InsPos) 01ax - 23aq
wt amplicon start Chr5:15776025
wt amplicon end Chr5:15776775
wt amplicon size 751 bp
Locus specific primer 23aq: 5'-GATGCTGTCTTGGTATTGTTGAGA-3'
2nd locus specific primer 01ax: 5'-GAGAAGATCATGTGATGGTGAAGA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11