Genotyping and primer details


Genotyping and primer details for 369A05 - At4g31760
Line ID 369A05
Locus At4g31760 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:15368677 of line 369A05 Chr4:15368677
   
Insertion allele amplicon 8474 - GE05
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer GE05: 5'-CAGGTAACACGCACTAATTACGAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:15369505
   
wt amplicon (over InsPos) 41rd - GE05
wt amplicon start Chr4:15368213
wt amplicon end Chr4:15369505
wt amplicon size 1293 bp
Locus specific primer GE05: 5'-CAGGTAACACGCACTAATTACGAA-3'
2nd locus specific primer 41rd: 5'-CAGACTATCCAAAATGGGACAAAT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11