Genotyping and primer details


Genotyping and primer details for 369B07 - At2g24990
Line ID 369B07
Locus At2g24990 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:10625804 of line 369B07 Chr2:10625804
   
Insertion allele amplicon 8474 - 17ve
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 17ve: 5'-TCCTCCGGTAGTAGAATACGAAGA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:10625326
   
wt amplicon (over InsPos) 18vu - 17ve
wt amplicon start Chr2:10625326
wt amplicon end Chr2:10626570
wt amplicon size 1245 bp
Locus specific primer 17ve: 5'-TCCTCCGGTAGTAGAATACGAAGA-3'
2nd locus specific primer 18vu: 5'-ACACACCCACAAAGAATTATAGGC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11