Genotyping and primer details


Genotyping and primer details for 370G06 - At2g39280
Line ID 370G06
Locus At2g39280 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:16406067 of line 370G06 Chr2:16406067
   
Insertion allele amplicon 8474 - 19uj
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 19uj: 5'-TCTTTCAACTATCACCGCCTCTAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:16406706
   
wt amplicon (over InsPos) 50vc - 19uj
wt amplicon start Chr2:16405590
wt amplicon end Chr2:16406706
wt amplicon size 1117 bp
Locus specific primer 19uj: 5'-TCTTTCAACTATCACCGCCTCTAT-3'
2nd locus specific primer 50vc: 5'-ATCAGTTGAGGCTAAGGAATTCAA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11