Genotyping and primer details


Genotyping and primer details for 374E12 - At4g20450
Line ID 374E12
Locus At4g20450 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:11026451 of line 374E12 Chr4:11026451
   
Insertion allele amplicon 8474 - EQ40
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer EQ40: 5'-TGGTCCCATTTAATCCAAAGTTAG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:11026040
   
wt amplicon (over InsPos) KF51 - EQ40
wt amplicon start Chr4:11026040
wt amplicon end Chr4:11026837
wt amplicon size 798 bp
Locus specific primer EQ40: 5'-TGGTCCCATTTAATCCAAAGTTAG-3'
2nd locus specific primer KF51: 5'-ATATAAACACCACCACCCCTGTAA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11