Genotyping and primer details


Genotyping and primer details for 374F11 - At5g24060
Line ID 374F11
Locus At5g24060 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:8131507 of line 374F11 Chr5:8131507
   
Insertion allele amplicon 8474 - 41uv
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 41uv: 5'-ATTCTGCTGCATGGTGTTT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:8131827
   
wt amplicon (over InsPos) 45vx - 41uv
wt amplicon start Chr5:8130933
wt amplicon end Chr5:8131827
wt amplicon size 895 bp
Locus specific primer 41uv: 5'-ATTCTGCTGCATGGTGTTT-3'
2nd locus specific primer 45vx: 5'-GAGAGGCTTTATCGTAACCTGATG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11