Genotyping and primer details


Genotyping and primer details for 377E05 - At1g79610
Line ID 377E05
Locus At1g79610 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:29956088 of line 377E05 Chr1:29956088
   
Insertion allele amplicon 8474 - 06ht
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 06ht: 5'-CAATGAATCTCAACTAGGCAACAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:29956418
   
wt amplicon (over InsPos) 07io - 06ht
wt amplicon start Chr1:29955751
wt amplicon end Chr1:29956418
wt amplicon size 668 bp
Locus specific primer 06ht: 5'-CAATGAATCTCAACTAGGCAACAA-3'
2nd locus specific primer 07io: 5'-AAGGAACCAGAATTCACTTAGCAA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11