Genotyping and primer details


Genotyping and primer details for 377H02 - At2g35585
Line ID 377H02
Locus At2g35585 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:14937581 of line 377H02 Chr2:14937581
   
Insertion allele amplicon 8474 - GS01
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer GS01: 5'-TCTGTTAGTTGTGTGCTTGAGCTT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:14937020
   
wt amplicon (over InsPos) 21rd - GS01
wt amplicon start Chr2:14937020
wt amplicon end Chr2:14938038
wt amplicon size 1019 bp
Locus specific primer GS01: 5'-TCTGTTAGTTGTGTGCTTGAGCTT-3'
2nd locus specific primer 21rd: 5'-TAGCTCTTAATTGGGAAACATTGG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11