Genotyping and primer details


Genotyping and primer details for 379C05 - At2g21940
Line ID 379C05
Locus At2g21940 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:9351262 of line 379C05 Chr2:9351262
   
Insertion allele amplicon 8474 - 19ve
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 19ve: 5'-TAAAAATCCTTTCCCTTTTACGGT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:9350697
   
wt amplicon (over InsPos) 75wa - 19ve
wt amplicon start Chr2:9350697
wt amplicon end Chr2:9351974
wt amplicon size 1278 bp
Locus specific primer 19ve: 5'-TAAAAATCCTTTCCCTTTTACGGT-3'
2nd locus specific primer 75wa: 5'-AAATTCTCTCCGTGATGAACAAAT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11