Genotyping and primer details


Genotyping and primer details for 380B09 - At1g27000
Line ID 380B09
Locus At1g27000 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:9374038 of line 380B09 Chr1:9374038
   
Insertion allele amplicon 8474 - KP75
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer KP75: 5'-TTTCAACAAAACAGCAGAATTCAC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:9374831
   
wt amplicon (over InsPos) 12dw - KP75
wt amplicon start Chr1:9373617
wt amplicon end Chr1:9374831
wt amplicon size 1215 bp
Locus specific primer KP75: 5'-TTTCAACAAAACAGCAGAATTCAC-3'
2nd locus specific primer 12dw: 5'-TGATCCATTTTTGGTCTGTTACAA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11