Genotyping and primer details


Genotyping and primer details for 380E11 - At5g42570
Line ID 380E11
Locus At5g42570 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:17022114 of line 380E11 Chr5:17022114
   
Insertion allele amplicon 8474 - 26lm
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 26lm: 5'-AACAATGATCCACCTCCTTTACAC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:17022501
   
wt amplicon (over InsPos) 01mc - 26lm
wt amplicon start Chr5:17021720
wt amplicon end Chr5:17022501
wt amplicon size 782 bp
Locus specific primer 26lm: 5'-AACAATGATCCACCTCCTTTACAC-3'
2nd locus specific primer 01mc: 5'-CTCAAAACCTCTGTTCTGCTTCTT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11