Genotyping and primer details


Genotyping and primer details for 382C01 - At2g25350
Line ID 382C01
Locus At2g25350 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:10798415 of line 382C01 Chr2:10798415
   
Insertion allele amplicon 8474 - AC83
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer AC83: 5'-TCGTGAACTATTCCAAGGAACTCT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:10798052
   
wt amplicon (over InsPos) 28si - AC83
wt amplicon start Chr2:10798052
wt amplicon end Chr2:10798913
wt amplicon size 862 bp
Locus specific primer AC83: 5'-TCGTGAACTATTCCAAGGAACTCT-3'
2nd locus specific primer 28si: 5'-AGAATTTGGGTGAACTAGAGACGA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11