Genotyping and primer details


Genotyping and primer details for 383H09 - At4g18290
Line ID 383H09
Locus At4g18290 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:10117736 of line 383H09 Chr4:10117736
   
Insertion allele amplicon 8474 - 29kh
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 29kh: 5'-TTGTGCAGGATTTTACTGTCTACG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:10117367
   
wt amplicon (over InsPos) 06ky - 29kh
wt amplicon start Chr4:10117367
wt amplicon end Chr4:10118127
wt amplicon size 761 bp
Locus specific primer 29kh: 5'-TTGTGCAGGATTTTACTGTCTACG-3'
2nd locus specific primer 06ky: 5'-GGATATGCTTCCTGTGAATGAAAT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11