Genotyping and primer details


Genotyping and primer details for 386E04 - At4g02160
Line ID 386E04
Locus At4g02160 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:954943 of line 386E04 Chr4:954943
   
Insertion allele amplicon 8474 - 53ra
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 53ra: 5'-AACGTCATAAACCTAATGCCAAAC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:954399
   
wt amplicon (over InsPos) 36rt - 53ra
wt amplicon start Chr4:954399
wt amplicon end Chr4:955470
wt amplicon size 1072 bp
Locus specific primer 53ra: 5'-AACGTCATAAACCTAATGCCAAAC-3'
2nd locus specific primer 36rt: 5'-AAACGACAATGTTACGGTACTTCC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11