Genotyping and primer details


Genotyping and primer details for 386H06 - At4g09340
Line ID 386H06
Locus At4g09340 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:5926860 of line 386H06 Chr4:5926860
   
Insertion allele amplicon 8474 - MN04
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer MN04: 5'-ATCCCATCGATAAATTACACAAGC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:5926524
   
wt amplicon (over InsPos) 04jb - MN04
wt amplicon start Chr4:5926524
wt amplicon end Chr4:5927297
wt amplicon size 774 bp
Locus specific primer MN04: 5'-ATCCCATCGATAAATTACACAAGC-3'
2nd locus specific primer 04jb: 5'-AATAGTTTCAGAATCGCGAGACAT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11