Genotyping and primer details


Genotyping and primer details for 388B09 - At4g37900
Line ID 388B09
Locus At4g37900 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:17822374 of line 388B09 Chr4:17822374
   
Insertion allele amplicon 8474 - MO10
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer MO10: 5'-TCATCATATTACCACATCCTCCAC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:17821793
   
wt amplicon (over InsPos) 49dy - MO10
wt amplicon start Chr4:17821793
wt amplicon end Chr4:17822956
wt amplicon size 1164 bp
Locus specific primer MO10: 5'-TCATCATATTACCACATCCTCCAC-3'
2nd locus specific primer 49dy: 5'-TTTATCGTTTTCGGAGTTTCTTTC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11