Genotyping and primer details


Genotyping and primer details for 388H10 - At5g04220
Line ID 388H10
Locus At5g04220 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:1156376 of line 388H10 Chr5:1156376
   
Insertion allele amplicon 8474 - 25aq
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 25aq: 5'-GAATGGAACGAACACTTTAAGCTC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:1156928
   
wt amplicon (over InsPos) 14ej - 25aq
wt amplicon start Chr5:1155747
wt amplicon end Chr5:1156928
wt amplicon size 1182 bp
Locus specific primer 25aq: 5'-GAATGGAACGAACACTTTAAGCTC-3'
2nd locus specific primer 14ej: 5'-CAATCAATATATCACCAGACCACG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11