Genotyping and primer details


Genotyping and primer details for 390H07 - At1g79640
Line ID 390H07
Locus At1g79640 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:29970363 of line 390H07 Chr1:29970363
   
Insertion allele amplicon 8474 - 18dl
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 18dl: 5'-TTTTGGAGATGAAATCCAGGTATC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:29969903
   
wt amplicon (over InsPos) 07ec - 18dl
wt amplicon start Chr1:29969903
wt amplicon end Chr1:29970990
wt amplicon size 1088 bp
Locus specific primer 18dl: 5'-TTTTGGAGATGAAATCCAGGTATC-3'
2nd locus specific primer 07ec: 5'-CGTTTGATGAAGTCGTTGCTATTA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11