Genotyping and primer details


Genotyping and primer details for 394F06 - At5g66480
Line ID 394F06
Locus At5g66480 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:26546032 of line 394F06 Chr5:26546032
   
Insertion allele amplicon 8474 - 21fp
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 21fp: 5'-GTTTATCCGTTATCGACTCTCTCG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:26546593
   
wt amplicon (over InsPos) 32sq - 21fp
wt amplicon start Chr5:26545788
wt amplicon end Chr5:26546593
wt amplicon size 806 bp
Locus specific primer 21fp: 5'-GTTTATCCGTTATCGACTCTCTCG-3'
2nd locus specific primer 32sq: 5'-TCAGCTCTCTCGAAATCTTCTACC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11