Genotyping and primer details


Genotyping and primer details for 396E06 - At1g23790
Line ID 396E06
Locus At1g23790 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:8411253 of line 396E06 Chr1:8411253
   
Insertion allele amplicon 8474 - 20ve
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 20ve: 5'-CACTTGTGCCTTTACATTTCTTCA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:8411765
   
wt amplicon (over InsPos) 44vx - 20ve
wt amplicon start Chr1:8410476
wt amplicon end Chr1:8411765
wt amplicon size 1290 bp
Locus specific primer 20ve: 5'-CACTTGTGCCTTTACATTTCTTCA-3'
2nd locus specific primer 44vx: 5'-TGAGAGTTCGAGACCTAGAAGAGG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11